Influence of the dental topical application of a nisin-biogel in the oral microbiome of dogs: a pilot study

Periodontal disease (PD) is one of the most widespread inflammatory diseases in dogs. This initiated by a polymicrobial biofilm teeth surface (dental plaque), leading to local response, with gingivitis and/or several degrees periodontitis. For instance, prevention bacterial dental plaque formation and its removal are essential steps PD control. Recent research revealed that antimicrobial peptide nisin incorporated delivery system guar gum (biogel) can inhibit eradicate bacteria from canine plaque, being promising compound for onset However, no information available regarding effect on dog’s oral microbiome. In this pilot study, influence nisin-biogel diversity microbiome was evaluated using next generation sequencing (NGS), aiming access viability be used long-term experiment Composite toothbrushing samples supragingival two dogs were collected at three timepoints: T1—before any application animals’ surface; T2—one hour after nisin-biogel; T3—one total applications nisin-biogel, each 48 hours. After that, microbial profiling performed NGS V3V4 16s rRNA region. only cavity dogs, statistically significant reduction observed (T2) as well some species potentially related distinct stages PD, when compared before (T1). (T3), recovery detected. conclusion, may A observed. study will help design controlled vivo clinical trial evaluate progression periodontal indices evolution period.